attune nxt software (Thermo Fisher)
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attune nxt software
Attune Nxt Software, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/attune+nxt+software/attune+nxt+software/pm40623717-94-30-34
Average 90 stars, based on 1 article reviews
Attune Nxt Software, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/attune+nxt+software/attune+nxt+software/pm40623717-94-30-34
Average 90 stars, based on 1 article reviews
attune nxt software - by Bioz Stars,
2026-10
90/100 stars
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Cytometry:Article Title: Multiple lesion inductions intensify central sensitization driven by neuroinflammation in a mouse model of endometriosis Article Snippet: The cells were blocked on ice for 20 min with Fc Block anti-CD16/CD32 (ThermoFisher) and stained with fluorochrome-conjugated monoclonal antibodies for 1 hour (Supplementary Table S1). .. Samples (n=5/group) were acquired with the Attune NxT Acoustic Focusing Cytometer using Article Title: Loss of DNA replication fork protection by TIMELESS degradation supports oncogene-induced senescence. Article Snippet: Induction of SAβG was quantified either by a senescence β-galactosidase X-gal staining kit (Cell Signaling Technology) or CellEventTM senescence green flow cytometry kit (Thermo Fisher) following the manufacturer’s protocols. .. Flow cytometry samples were analyzed by the Attune NxT acoustic focusing cytometer (Thermo Fisher) and Article Title: Oncolytic HSV-IL27 expression improves CD8 T cell function and therapeutic activity in syngeneic glioma models. Article Snippet: For intracellular staining, cells were fixed and permeabilized (eBioscience, Foxp3 Transcription Factor Fixation/Permeabilization) following the manufacturer’s intracellular staining protocol then incubated with intracellular antibodies overnight at 4°C. .. Samples were washed and resuspended in flow cytometry staining buffer for acquisition on a Cytek Aurora spectral flow cytometer using SpectralFlo software V.3.1.0 or Attune NxT Acoustic focusing cytometer using Article Title: Efficiency of genome editing using modified single-stranded oligodeoxyribonucleotides in human cells. Article Snippet: The plate was returned to the incubator for 24 h. The supernatant was removed, and the cells were transferred to a 100 mm dish (Eppendorf, Hamburg, Germany) and continuously cultured for 4 days until the cells reached confluence (reach approximately 90 ~ 100%). .. EGFP-positive cells were quantified using an Attune NxT Acoustic Focusing Cytometer (Thermo Fisher Scientific, Inc.) and analyzed with Software:Article Title: Multiple lesion inductions intensify central sensitization driven by neuroinflammation in a mouse model of endometriosis Article Snippet: The cells were blocked on ice for 20 min with Fc Block anti-CD16/CD32 (ThermoFisher) and stained with fluorochrome-conjugated monoclonal antibodies for 1 hour (Supplementary Table S1). .. Samples (n=5/group) were acquired with the Attune NxT Acoustic Focusing Cytometer using Article Title: Pooling umbilical cord-mesenchymal stromal cells derived from selected multiple donors reduces donor-dependent variability and improves their immunomodulatory properties Article Snippet: .. The acquisitions were performed with Attune NxT TM Thermofisher ® Flow Cytometer and analyzes were performed using Article Title: Viability of Partial Heart Transplant Grafts During Prolonged Cold Preservation Suggests That Longer Donor Cold Chain Logistics May Be Feasible. Article Snippet: Background: Partial heart transplantation (PHT) is a new type of transplant that delivers growing heart valve implants for children.. However, the acceptable ischemia time for PHTs remains unexplored.. Therefore, the ischemia time for PHTs is empirically limited to orthotopic heart transplant (OHT) ischemia time of 4–6 h because the valves contained in OHTs are known to grow. Article Title: Loss of DNA replication fork protection by TIMELESS degradation supports oncogene-induced senescence. Article Snippet: Induction of SAβG was quantified either by a senescence β-galactosidase X-gal staining kit (Cell Signaling Technology) or CellEventTM senescence green flow cytometry kit (Thermo Fisher) following the manufacturer’s protocols. .. Flow cytometry samples were analyzed by the Attune NxT acoustic focusing cytometer (Thermo Fisher) and Article Title: Oncolytic HSV-IL27 expression improves CD8 T cell function and therapeutic activity in syngeneic glioma models. Article Snippet: For intracellular staining, cells were fixed and permeabilized (eBioscience, Foxp3 Transcription Factor Fixation/Permeabilization) following the manufacturer’s intracellular staining protocol then incubated with intracellular antibodies overnight at 4°C. .. Samples were washed and resuspended in flow cytometry staining buffer for acquisition on a Cytek Aurora spectral flow cytometer using SpectralFlo software V.3.1.0 or Attune NxT Acoustic focusing cytometer using Article Title: Glycogen-inspired trimannosylated serum albumin nanocarriers for targeted delivery of toll-like receptor 7/8 agonists to immune cells and liver. Article Snippet: Nanocarriers can improve the therapeutic efficiency of small molecule immunomodulators or inhibitors, which is important for immunotherapy of liver diseases or cancer.. Macromolecular protein carriers, such as human serum albumin (HSA), could provide better penetration compared to large nanoparticles (>50 nm) but are hampered by systemic biodistribution.. To overcome these limitations, inspired by the natural glycogen structure, we have designed the HSA nanocarrier (<40 nm) consisting of multiple trimannose (TM) ligands attached to the protein surface, to target mannose or dendritic cell-specific intercellular adhesion molecule-3-grabbing non-integrin (DCSIGN) receptors in immune cells or immunological organs such as the liver. Article Title: Efficiency of genome editing using modified single-stranded oligodeoxyribonucleotides in human cells. Article Snippet: The plate was returned to the incubator for 24 h. The supernatant was removed, and the cells were transferred to a 100 mm dish (Eppendorf, Hamburg, Germany) and continuously cultured for 4 days until the cells reached confluence (reach approximately 90 ~ 100%). .. EGFP-positive cells were quantified using an Attune NxT Acoustic Focusing Cytometer (Thermo Fisher Scientific, Inc.) and analyzed with Flow Cytometry:Article Title: Pooling umbilical cord-mesenchymal stromal cells derived from selected multiple donors reduces donor-dependent variability and improves their immunomodulatory properties Article Snippet: .. The acquisitions were performed with Attune NxT TM Thermofisher ® Flow Cytometer and analyzes were performed using Article Title: Loss of DNA replication fork protection by TIMELESS degradation supports oncogene-induced senescence. Article Snippet: Induction of SAβG was quantified either by a senescence β-galactosidase X-gal staining kit (Cell Signaling Technology) or CellEventTM senescence green flow cytometry kit (Thermo Fisher) following the manufacturer’s protocols. .. Flow cytometry samples were analyzed by the Attune NxT acoustic focusing cytometer (Thermo Fisher) and Article Title: Oncolytic HSV-IL27 expression improves CD8 T cell function and therapeutic activity in syngeneic glioma models. Article Snippet: For intracellular staining, cells were fixed and permeabilized (eBioscience, Foxp3 Transcription Factor Fixation/Permeabilization) following the manufacturer’s intracellular staining protocol then incubated with intracellular antibodies overnight at 4°C. .. Samples were washed and resuspended in flow cytometry staining buffer for acquisition on a Cytek Aurora spectral flow cytometer using SpectralFlo software V.3.1.0 or Attune NxT Acoustic focusing cytometer using Article Title: Glycogen-inspired trimannosylated serum albumin nanocarriers for targeted delivery of toll-like receptor 7/8 agonists to immune cells and liver. Article Snippet: Nanocarriers can improve the therapeutic efficiency of small molecule immunomodulators or inhibitors, which is important for immunotherapy of liver diseases or cancer.. Macromolecular protein carriers, such as human serum albumin (HSA), could provide better penetration compared to large nanoparticles (>50 nm) but are hampered by systemic biodistribution.. To overcome these limitations, inspired by the natural glycogen structure, we have designed the HSA nanocarrier (<40 nm) consisting of multiple trimannose (TM) ligands attached to the protein surface, to target mannose or dendritic cell-specific intercellular adhesion molecule-3-grabbing non-integrin (DCSIGN) receptors in immune cells or immunological organs such as the liver. Staining:Article Title: Oncolytic HSV-IL27 expression improves CD8 T cell function and therapeutic activity in syngeneic glioma models. Article Snippet: For intracellular staining, cells were fixed and permeabilized (eBioscience, Foxp3 Transcription Factor Fixation/Permeabilization) following the manufacturer’s intracellular staining protocol then incubated with intracellular antibodies overnight at 4°C. .. Samples were washed and resuspended in flow cytometry staining buffer for acquisition on a Cytek Aurora spectral flow cytometer using SpectralFlo software V.3.1.0 or Attune NxT Acoustic focusing cytometer using other:Article Title: The critical role of PSAC channel in malaria parasite survival is driven home by phenotypic screening under relevant nutrient levels. Article Snippet: Cell lines and culture conditions P. falciparum strains were obtained from BEI Resources and immediately expanded, and frozen in aliquots: Dd2, 3D7A, 7G8, W2F6, NF54 and HB3. |